ELECTRON MICROSCOPY FACILITY

Scanning electron microscope FEI Quanta 250
- scanning and analysis of biological and non-biological surfaces.
- quantitative and qualitative microanalysis on a certain delimited surface;
- detection of chemical elements, from composition analyses on rough samples, including the comparison of results to standards in the database;
- combined analysis based on dispersed energy and wavelength;
- acquisition and processing of color images and analysis of morphological characters;
- measurements of depth, area and volume in studied samples;
- detection of structure for different compositions of ferrous and non-ferrous materials.
- morphological and compositional characterization of powders;
- analysis of pedological and biological materials for the control of environmental
pollution;
- analysis and testing of different substances and ingredients from drugs and foods.

 

Transmission electron microscope FEI Tecnai 12, Biotwin
Auxiliary equipment: Ultramicrotome Leica UC7, Sputter-coater Agar, gold film deposition
- structure and ultrastructure analyses in biological samples from animal and vegetal tissue, fungi, bacteria, viruses.
- cross-sections through biological and non-biological samples.
- examination of biological and nonbiological samples by negative staining.
- electron tomography and 3D reconstruction of structures, ultrastructures.


HISTOPATHOLOGY, OPTICAL, FLUORESCENCE AND CONFOCAL MICROSCOPY LABORATORY

Equipment: MicroTec and Leica Microtomes, Paraffin thermostat, Cryostat SLEE- MAINZ- MNT, Microscope Olympus BX43 with florescence module and Software Cell Dimension, Confocal Microscope Leica TCS SP8
- preparation of extemporaneous and permanent tissue samples
- histopathological, histochemical and immunohistochemical investigation on animal tissue samples
(optical/fluorescence microscopy, confocal microscopy)

- applicability areas: toxicology, phythotherapy, tissue engineering, nanotechnologies, cancer biology, neurobiology, etc.


FLOW CYTOMETRY LABORATORY

 

Equipment: Cytometer Beckman Coulter Cyromics FC 500
- Immunophenotyping
- Membrane potential, Membrane fluidity, Monitoring of cell electropermeability
- Cell viability assay (volume and morphological complexity of cells)
- Apoptosis (quantification, DNA degradation, mitochondrial membrane potential, changes in membrane
permeability)
- Cell surface antigens, intracellular and nuclear


LIQUID CHROMATOGRAPHY LABORATORY

 

Equipment: UltiMate 3000 Standard HPLC Systems

- Analysis of complex mixtures
- Purification of chemical compounds
- Development of chemical compound synthesis processes

- Isolation of natural products

-Separation of bioactive compounds: alkaloids, vitamins, flavonoids, etc.

 

PLANT BIOTECHNOLOGIES LABORATORY

 

- plant in vitro cultures
- in vitro micropropagation
- in vitro conservation (living collection)
- alternative LED illumination of phythoinocula

 

CYTOGENETICS AND MOLECULAR GENETICS LABORATORY

 

- DNA polymorphism in genetic predispositions for different pathological
situations;

- RT-PCR, QRT-PCR, HRM for gene mutations in human pathology and microdeletions of the Y chromosome
- Classic and molecular cytogenetics (FISH) in hereditary diseases
- Chromosomal rearrangements – oncology

 

ECOLOGY AND ENVIRONMENTAL PROTECTION LABORATORY

 

- Study and monitoring of vegetal and animal biodiversity
- Assessment of habitat quality
- Assessment of the impact of land use on biodiversity
- Study of invasive species
- Assessment of the impact of transport infrastructure on habitat connectivity,
- Ecological and economic modeling for biodiversity
conservation,
- Establishing biodiversity management strategies,
- Biometrics
- Ecotoxicology

 

 

SUPPORTING STRUCTURES

 

Animal facility

Authorized by the Sanitary Veterinary and Food Safety Directorate under Authorization no. 597/12.12.2013

Total capacity is 1,600 formice and 400 for rats, under special safety conditions.
Allows changing the bedding under safety condi-tions for operator and ani-mals

Biochemical analyses (AST, ALT, GGT, LDH, ALP, CK-MB, cholesterol, triglycerides, total proteins, total lipids, uric acid, cholinesterase, albumin, alpha-amylase, phosphorus and potassium, apolipoproteins,

bile acids, lipase, complement, etc.) and hematological analyses.
-Inhalational anesthesia, oral (gavage), intraperitoneal, intramuscular, intravenous administrations
-Organ perfusion, organ harvesting
- Surgeries (e.g. creation of bone defects and replacement with scaffold), clinical monitoring
- Analysis of fluorescence, luminescence, radioisotope and X-ray images and analysis of bone densitometry

Characteristics: 4 MP camera, luminescence sensitivity<650 photons/sec/cm2 and <50 photons/sec/cm2/sr; pixels CCD 2048 x 2048, FOV 7.2 x 7.2 cm to 20 x 20 cm; fluorescence: excitation 410 nm – 760 nm, emission 535 nm – 830 nm, 6 filters X ray: resolution > 18 lp/mm Applications: in vivo studies of pharmacodynamics; use, development and validation of samples and biomark- ers, quantification of structural changes in bone and soft tissues; recording of cell migration in vitro and in vivo; co-recording of functional images (optical and radioisotopic) with anatomical images in x-ray.

 

 

MACEA BOTANICAL GARDEN

 

The Macea Botanical Garden has anarea of 21.5 ha; 3810 taxa (around 2,100of which are ligneous) and was foundedin 1968.
- founding member of Botanical GardenAssociation of Romania (AGBR)
- member of Botanic Gardens Conservation
International (BGCI)
- ongoing exchanges of biological material with over 225 botanical gardens andarboreta across the world

The tree collection comprises2,579 taxa, of which 2,024 species, 73 subspecies, 156 varieties, 27 forms and 299 cultivars. Gymnosperms are represented by 249 taxa, of which 132 species,4 subspecies, 13 varieties and 100 cultivars, and Angiosperms by 2330 taxa, with1892 species, 69 subspecies, 143 varieties, 27 forms and 199 cultivars. Spontaneous or cultivated dendrotaxa in Macea pertain to 282 genera. Among the best represented genera are Cotoneaster (with 123 taxa), Crataegus(112 taxa), Lonicera (101 taxa), Acer(100), Prunus (87), Betula (71), Quercus(60), Berberis (58), Viburnum (57), andamong the conifers Pinus (42 taxa) andJuniperus (37), Chamaecyparis (28) andThuja (26).
It also comprises:
- Seed bank – Gene bank - it currentlyincludes over 2,500 samples harvested since 2013 and until now. The areareserved for the collection is 110 m², equipped with all that is necessary to process fruit, as well as to condition andpreserve seeds
- Botanic Museum and Herbarium
- two plant biology laboratories





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